ImmunoCheck
Comprehensive immunogenicity and developability assessment for VHH nanobodies. MHC-II epitope prediction, humanization scoring, and physicochemical profiling — in one API call.
No API key required. Free for academic and commercial use.
Everything You Need to Assess Your Antibody
Three complementary analyses in a single endpoint. Built for bioinformaticians and antibody engineers.
MHC-II Epitope Prediction
Screen against 8 HLA-DRB1 alleles (EMA/FDA reference set). Identifies strong and weak binders, hotspot positions, and epitope density.
Humanization Scoring
Compares against 26 IGHV germlines. T20 score, framework identity, VHH hallmark residue analysis at positions 37, 44, 45, 47.
Developability Profiling
Full physicochemical profiling: MW, pI, GRAVY, instability index, aggregation hotspots, chemical liabilities (deamidation, oxidation, isomerization).
Fast & Lightweight
Results in under 3 seconds (without epitopes). No GPU required. Pure algorithmic analysis.
No API Key Required
Completely free to use. Rate limited to 5 requests/minute per IP. No registration, no sign-up.
OpenAPI / Swagger
Full interactive documentation at /docs. Auto-generated client libraries. JSON responses.
Analyze Your Sequence
Paste a VHH or antibody sequence below and get instant results from the live API.
Integrate in Minutes
One REST endpoint. No SDK needed. Works with any language.
import requests
response = requests.post(
"https://api.jfinnova.es/api/v1/immunocheck",
json={
"sequence": "QVQLVESGGGLVQPGGSLRL...",
"format": "VHH",
"include_epitopes": True
}
)
data = response.json()
print(f"Risk: {data['overall_risk']} ({data['risk_score']}/10)")
print(f"Humanization: {data['humanization']['humanization_grade']}")
print(f"Developability: {data['developability']['developability_grade']}")